Development of a Rapid Loop-Mediated Isothermal Amplification Assay for the Detection of Dothistroma septosporum
نویسندگان
چکیده
A Loop-Mediated Isothermal Amplification (LAMP) assay was developed for the detection of pine pathogen Dothistroma septosporum (G. Dorog.) M. Morelet. The specificity LAMP tested using a selection needle fungi, including pini Hulbary, and Lecanosticta acicola (Thüm.) Syd.; only D. DNA amplified by test. In terms sensitivity, able to detect as little 1 pg total DNA. This enables extracted from diseased host needles be rapidly presence relatively simple operate equipment away fully equipped molecular biology laboratory.
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ژورنال
عنوان ژورنال: Forests
سال: 2021
ISSN: ['1999-4907']
DOI: https://doi.org/10.3390/f12030362